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FM Dye Cycling at the Synapse: Comparing High Potassium Depolarization, Electrical and Channelrhodopsin Stimulation
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Chapters
- 00:04Title
- 00:42Larval Glue Dissection
- 02:36Electrical Stimulation: FM Dye Loading and Unloading
- 04:08Channelrhodopsin Stimulation: FM Dye Loading and Unloading
- 05:21Fluorescence Quantification
- 06:12Results: Synaptic Ultrastructure with FM Dye Photoconversion to Mark Vesicles
- 07:22Conclusion
Synaptic vesicle (SV) cycling is the core mechanism of intercellular communication at neuronal synapses. FM dye uptake and release are the primary means of quantitatively assaying SV endo- and exocytosis. Here, we compare all the stimulation methods to drive FM1-43 cycling at the Drosophila neuromuscular junction (NMJ) model synapse.
