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Database-guided Flow-cytometry for Evaluation of Bone Marrow Myeloid Cell Maturation
JoVE Journal
Medicine
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JoVE Journal Medicine
Database-guided Flow-cytometry for Evaluation of Bone Marrow Myeloid Cell Maturation
DOI:

12:05 min

November 03, 2018

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Chapters

  • 00:04Title
  • 01:07Bone Marrow (BM) Sample Preparation
  • 02:50Data Acquisition
  • 03:53Data Analysis
  • 07:48BM Myeloid Compartment Maturation Evaluation
  • 10:03Results: Representative Flow Cytometry Analysis of BM Cell Populations in a del(7) MDS Case
  • 10:33Conclusion

Summary

Automatic Translation

The MDS diagnosis is difficult in the absence of morphological criteria or non-informative cytogenetics. MFC could help refine the MDS diagnostic process. To become useful for clinical practice, the MFC analysis must be based on parameters with sufficient specificity and sensitivity, and data should be reproducible between different operators.

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