Journal
/
Developmental Biology
/
A Hyperandrogenic Mouse Model to Study Polycystic Ovary Syndrome
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
A Hyperandrogenic Mouse Model to Study Polycystic Ovary Syndrome

A Hyperandrogenic Mouse Model to Study Polycystic Ovary Syndrome

DOI:
10.3791/58379-v

•

08:20 min

•

October 02, 2018

•

, , , , , ,
1Department of Pediatrics,Johns Hopkins University School of Medicine, 2Department of Health,Beijing Military General Hospital, 3Southern Medical University, 4Department of Molecular and Cellular Physiology,Johns Hopkins University School of Medicine, 5Department of Gynecology and Obstetrics,Johns Hopkins University School of Medicine

Chapters

  • 00:04Title
  • 00:47Dihydrotestosterone (DHT) Pellet Preparation
  • 02:04DHT Insertion and Replacement
  • 04:56Reproductive Profile Assessment of Female Offspring from Chronically DHT-inserted Dams
  • 06:27Results: Representative Mouse PCOS Model Analyses
  • 07:28Conclusion

Summary

Automatic Translation

Automatic Translation

We describe the development of a lean PCOS-like mouse model with dihydrotestosterone pellet to study the pathophysiology of PCOS and the offspring from these PCOS-like dams.

Tags

Keywords: Polycystic Ovary SyndromePCOSHyperandrogenismDihydrotestosteroneDHTMouse ModelSilicone TubingPellet ImplantationSurgical ProcedureAndrogen Exposure

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/58379?article_type=v

Generating CRISPR/Cas9 Mediated Monoallelic Deletions to Study Enhancer Function in Mouse Embryonic Stem Cells
check_url/58379?article_type=v

Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos
check_url/58379?article_type=v

Isolation and Characterization of Primary Rat Valve Interstitial Cells: A New Model to Study Aortic Valve Calcification
check_url/58379?article_type=v

Whole-mount Confocal Microscopy for Adult Ear Skin: A Model System to Study Neuro-vascular Branching Morphogenesis and Immune Cell Distribution
check_url/58379?article_type=v

Organotypic Culture Method to Study the Development Of Embryonic Chicken Tissues
check_url/58379?article_type=v

Ovarian Tissue Culture to Visualize Phenomena in Mouse Ovary
check_url/58379?article_type=v

A Mouse Distraction Osteogenesis Model
check_url/58379?article_type=v

Use of Hematopoietic Stem Cell Transplantation to Assess the Origin of Myelodysplastic Syndrome
check_url/58379?article_type=v

An In Vitro Model of a Parallel-Plate Perfusion System to Study Bacterial Adherence to Graft Tissues
check_url/58379?article_type=v

An In Vivo Method to Study Mouse Blood-Testis Barrier Integrity

Read Article