Journal
/
Immunology and Infection
/
Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction

Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction

DOI:
10.3791/58407-v

•

08:36 min

•

November 01, 2018

•

, , , , ,
1Department of Microbiology and Infection Control,Osaka Medical College

Chapters

  • 00:04Title
  • 01:06Synthesis of the DENV 3’UTR RNA Standard
  • 03:15Processing of Virus Samples for RT-qPCR
  • 04:19Real-time PCR Analysis
  • 06:01Results: Real-time Quantitative Polymerase Chain Reaction of Virus RNA
  • 07:58Conclusion

Summary

Automatic Translation

Automatic Translation

Real-time quantitative polymerase chain reaction analysis combined with reverse transcription (RT-qPCR) has been widely used to measure the level of RNA virus infections. Here we present a direct RT-qPCR assay, which does not require an RNA purification step, developed for the quantification of several RNA viruses, including dengue virus.

Tags

Dengue VirusRNA QuantificationReal-time PCRRNA StandardIn Vitro TranscriptionRNA PurificationViral RNA DetectionVirus ResearchDrug ScreeningClinical Diagnosis

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/58407?article_type=v

Detection of Infectious Virus from Field-collected Mosquitoes by Vero Cell Culture Assay
check_url/58407?article_type=v

Measuring Bacterial Load and Immune Responses in Mice Infected with Listeria monocytogenes
check_url/58407?article_type=v

Visualizing Dengue Virus through Alexa Fluor Labeling
check_url/58407?article_type=v

Affinity Purification of Influenza Virus Ribonucleoprotein Complexes from the Chromatin of Infected Cells
check_url/58407?article_type=v

Real-time Imaging of Myeloid Cells Dynamics in ApcMin/+ Intestinal Tumors by Spinning Disk Confocal Microscopy
check_url/58407?article_type=v

Measuring Attachment and Internalization of Influenza A Virus in A549 Cells by Flow Cytometry
check_url/58407?article_type=v

Neutrophil Isolation and Analysis to Determine their Role in Lymphoma Cell Sensitivity to Therapeutic Agents
check_url/58407?article_type=v

Determination of Biofilm Initiation on Virus-infected Cells by Bacteria and Fungi
check_url/58407?article_type=v

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
check_url/58407?article_type=v

Quantitative Polymerase Chain Reaction-based Analyses of Murine Intestinal Microbiota After Oral Antibiotic Treatment

Read Article