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An Optimized Rhizobox Protocol to Visualize Root Growth and Responsiveness to Localized Nutrients
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An Optimized Rhizobox Protocol to Visualize Root Growth and Responsiveness to Localized Nutrients

An Optimized Rhizobox Protocol to Visualize Root Growth and Responsiveness to Localized Nutrients

DOI:
10.3791/58674-v

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07:45 min

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October 22, 2018

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, ,
1Department of Plant Sciences,University of California at Davis, 2Department of Natural Resources and the Environment,University of New Hampshire

Chapters

  • 00:04Title
  • 00:27Assembly of the Rhizoboxes
  • 02:08Substrate Preparation for Treatment and Control Patches
  • 02:54Loading the Rhizobox with Substrate and Establishing Treatment and Control Patches
  • 05:02Seed Transplantation and Plant Growth
  • 06:19Results: Roots Proliferate in Treated Patches
  • 07:26Conclusion

Summary

Automatic Translation

Automatic Translation

Visualizing and measuring root growth in situ is extremely challenging. We present a customizable rhizobox method to track root development and proliferation over time in response to nutrient enrichment. This method is used to analyze maize genotypic differences in root plasticity in response to an organic nitrogen source.

Tags

RhizoboxRoot GrowthLocalized NutrientsRoot And Rhizosphere ScienceNon-invasive MeasurementsSoilAcrylic PanelsPolyester PaddingPolyethylene SheetsPVC FrameSoil-sand SubstrateNitrogen Source

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