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Immunology and Infection
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Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing

Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing

DOI:
10.3791/58804-v

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08:51 min

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March 15, 2019

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, , , , , , , , , , , ,
1Graduate School of Life and Environmental Sciences,University of Tsukuba, 2Center for Influenza Virus Research,National Institute of Infectious Diseases, 3School of Science and Technology,Kwansei Gakuin University, 4Department of Pathology,National Institute of Infectious Diseases, 5Division of Infectious Diseases Pathology, Department of Global Infectious Diseases,Tohoku University Graduate School of Medicine, 6Division of Molecular Pathobiology, Research Center for Zoonosis Control,Hokkaido University, 7Division of Collaboration and Education, Research Center for Zoonosis Control,Hokkaido University, 8Global Station for Zoonosis Control, GI-CoRE,Hokkaido University, 9Department of Immunology,National Institute of Infectious Diseases, 10Faculty of Life and Environmental Sciences,University of Tsukuba

Chapters

  • 00:04Title
  • 01:23Nucleic Acid Isolation from Immune Cells and Tissues
  • 03:04cDNA Synthesis and PCR Amplification
  • 04:58NGS Sequencing of Libraries
  • 05:33Results: Identification of Mouse and Human Antibody Repertoires
  • 08:11Conclusion

Summary

Automatic Translation

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Here, we describe protocols for the analysis and visualization of the structure and constitution of whole antibody repertoires. This involves the acquisition of vast sequences of antibody RNA using next-generation sequencing.

Tags

Antibody RepertoireNext-generation SequencingB Cell RepertoireImmune ResponseVaccine EfficacyImmunoglobulin AmplificationRNA Extraction

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