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Preparation of Poly(pentafluorophenyl acrylate) Functionalized SiO2 Beads for Protein Purification
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JoVE Journal Chemistry
Preparation of Poly(pentafluorophenyl acrylate) Functionalized SiO2 Beads for Protein Purification

Preparation of Poly(pentafluorophenyl acrylate) Functionalized SiO2 Beads for Protein Purification

DOI:
10.3791/58843-v

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08:51 min

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November 19, 2018

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, , , ,
1Department of Chemical and Biomolecular Engineering,Korea Advanced Institute of Science and Technology (KAIST), 2KI for Health Science and Technology (KIHST),Korea Advanced Institute of Science and Technology (KAIST)

Chapters

  • 00:04Title
  • 00:54Preparation of Poly(PFPA) Functionalized SiO2 Beads
  • 02:31Preparation of SiO2 Beads Grafted with PEG-Substituted Poly(PFPA)
  • 04:06Antibody Immobilization on Poly(PFPA) Grafted SiO2 Beads
  • 05:20Cell Lysis and Immunoprecipitation
  • 07:00Results: Analysis of Poly (pentafluorophenyl acrylate) Functionalized SiO2 Beads for Protein Purification
  • 08:09Conclusion

Summary

Automatic Translation

Automatic Translation

A protocol for the preparation of poly(pentafluorophenyl acrylate) (poly(PFPA)) grafted silica beads is presented. The poly(PFPA) functionalized surface is then immobilized with antibodies and used successfully for the protein separation through immunoprecipitation.

Tags

Poly(pentafluorophenyl Acrylate)SiO2 BeadsProtein PurificationAPTESPFPA BrushesAntibody ImmobilizationImmunopurificationBiomolecule ActivationAmine-reactiveDMSOPEG

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