Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Exploiting Live Imaging to Track Nuclei During…
JoVE Journal
Biology

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Biology
Exploiting Live Imaging to Track Nuclei During Myoblast Differentiation and Fusion
 

Exploiting Live Imaging to Track Nuclei During Myoblast Differentiation and Fusion

Article DOI: 10.3791/58888-v • 09:03 min • April 13th, 2019
April 13th, 2019 •
*1,2, *1,3, 1, 1, 1,2, *1,2, *1
1Chromatin Dynamics Unit, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS) San Raffaele Scientific Institute, 2Vita-Salute San Raffaele University, 3Department of Electronics, Information and Bioengineering, Politecnico di Milano
* These authors contributed equally

Chapters

Summary
Automatic Translation

Skeletal muscle differentiation is a highly dynamic process, which particularly relies on nuclear positioning. Here, we describe a method to track nuclei movements by live cell imaging during myoblast differentiation and myotube formation and to perform a quantitative characterization of nuclei dynamics by extracting information from automatic tracking.

Tags

Keywords: Live Cell Imaging Myoblast Differentiation Myofiber Formation Nuclear Positioning Primary Cell Isolation Cell Tracking Muscle Cell Isolation Satellite Cell Isolation
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter