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Evaluation of Colorectal Cancer Risk and Prevalence by Stool DNA Integrity Detection
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JoVE Journal Cancer Research
Evaluation of Colorectal Cancer Risk and Prevalence by Stool DNA Integrity Detection

Evaluation of Colorectal Cancer Risk and Prevalence by Stool DNA Integrity Detection

DOI:
10.3791/59426-v

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07:35 min

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June 08, 2020

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, , , ,
1Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, Meldola, Italy, 2Diatech Pharmacogenetics srl

Chapters

  • 00:04Introduction
  • 00:31Preparation of Positive Control, Standards, Spike-in DNA, and Clinical Samples
  • 01:52Amplification and Determination of the FL-DNA Value Using qPCR Easy PGX
  • 03:57Data Analysis
  • 05:36Results: Amplification Results for Target Genes and Internal Controls
  • 06:50Conclusion

Summary

Automatic Translation

Automatic Translation

The presented diagnostic FL-DNA kit is a time-saving and user-friendly method to determine the reliable probability of the presence of colorectal cancer lesions.

Tags

Colorectal CancerStool DNA Integrity DetectionNon-invasive ApproachCancer ScreeningPositive ControlStandard DNASpike-in DNAQPCRThermal ProfileAmplification MixturePrimersProbesNegative ControlSample DNAPositive Control DNAStandard Curve

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