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Toxicity Study of Zinc Oxide Nanoparticles in Cell Culture and in Drosophila melanogaster
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Immunology and Infection
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JoVE Journal Immunology and Infection
Toxicity Study of Zinc Oxide Nanoparticles in Cell Culture and in Drosophila melanogaster

Toxicity Study of Zinc Oxide Nanoparticles in Cell Culture and in Drosophila melanogaster

DOI:
10.3791/59510-v

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06:47 min

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September 19, 2019

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, , , ,
1NUS Environmental Research Institute & Department of Anatomy,National University of Singapore, 2NUS Environmental Research Institute & School of Public Health,National University of Singapore, 3Department of Civil and Environmental Engineering,National University of Singapore, 4Department of Anatomy,National University of Singapore

Chapters

  • 00:04Title
  • 00:42Fluorescence Activated Cell Sorting (FACS) Analysis on Lived/Fixed Cells
  • 02:12Exposure of ZnO NPs to Drosophila
  • 03:18Dissection of Fly and ROS Detection
  • 04:22Measuring Fluorescence using ImageJ Software
  • 05:03Results: Toxicity Study of ZnO NPs
  • 05:34Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a detailed protocol for evaluating the toxicological profiles of zinc oxide nanoparticles (ZnO NPs) in particular, the type of cell death in human MRC5 lung fibroblasts and ROS formation in the fruit fly Drosophila.

Tags

NanotoxicityCell DeathReactive Oxygen SpeciesFluorescence Activated Cell SortingDHE ProbeZinc Oxide NanoparticlesMRC5 Human Lung FibroblastsFITC Annexin VPropidium IodideFlow CytometryDrosophila MelanogasterFly Food

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