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Synthesis of a Deuterated Standard for the Quantification of 2-Arachidonoylglycerol in Caenorhabditis elegans
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1Instituto de Química Rosario (IQUIR-CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas,Universidad Nacional de Rosario, 2Laboratorio de Fisiología Microbiana, Instituto de Biología Molecular y Celular de Rosario (IBR), CONICET, Facultad de Ciencias Bioquímicas y Farmacéuticas,Universidad Nacional de Rosario, 3Departamento de Química Orgánica, Facultad de Ciencias Bioquímicas y Farmacéuticas,Universidad Nacional de Rosario
Chapters
- 00:00Title
- 02:131-AG-d5 Preparation
- 04:12Esterification Step
- 05:35Deprotection Step
- 06:34Bleaching Technique for Synchronizing C elegans Cultures
- 08:02Worm Sample Preparation
- 09:40Lipid Extraction
- 11:05Results
- 12:44Conclusion
This work describes a robust and straightforward method to detect and quantify the endocannabinoid 2-arachidonoylglycerol (2-AG) in C. elegans. An analytical deuterated standard us prepared and used for the quantification of 2-AG by isotopic dilution and liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS).
