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Immunology and Infection
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Induced Differentiation of M Cell-like Cells in Human Stem Cell-derived Ileal Enteroid Monolayers
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Induced Differentiation of M Cell-like Cells in Human Stem Cell-derived Ileal Enteroid Monolayers

Induced Differentiation of M Cell-like Cells in Human Stem Cell-derived Ileal Enteroid Monolayers

DOI:
10.3791/59894-v

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11:34 min

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July 26, 2019

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, , ,
1Program in Immunology,Sackler School of Graduate Biomedical Sciences, 2Department of Molecular Virology and Microbiology,Baylor College of Medicine, 3Department of Molecular Biology and Microbiology,Tufts University

Chapters

  • 00:04Title
  • 00:27Inducing M Cell Differentiation in Human Ileal Enteroid-derived Monolayers
  • 05:46Verifying M Cell Differentiation
  • 09:30Results: Immunofluorescence of M Cells in Human Ileal Enteroid-derived Monolayers
  • 11:05Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes how to induce the differentiation of M cells in human stem cell-derived ileal monolayers and methods to assess their development.

Tags

Keywords: M CellsIntestinal Stem CellsInduced DifferentiationIleal Enteroid MonolayersExtracellular MatrixTranswell MembranesEDTATrypsinCell DisaggregationSingle Cells

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