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Kinetic Screening of Nuclease Activity using Nucleic Acid Probes
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Biochemistry
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JoVE Journal Biochemistry
Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

DOI:
10.3791/60005-v

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06:52 min

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November 01, 2019

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, , , , ,
1Department of Physics, Chemistry and Biology,Linköping University, 2Wallenberg Centre for Molecular Medicine (WCMM), 3Nucleic Acids Technologies Laboratory (NAT-lab),Linköping University

Chapters

  • 00:05Title
  • 00:44Oligonucleotide Library Design and Preparation and Bacteria Culture
  • 02:07Nuclease Activity Assay Setup
  • 03:05Measurement Setup
  • 04:32Data Analysis
  • 05:12Results: Representative Kinetic Screening
  • 06:23Conclusion

Summary

Automatic Translation

Automatic Translation

Altered nuclease activity has been associated with different human conditions, underlying its potential as a biomarker. The modular and easy to implement screening methodology presented in this paper allows the selection of specific nucleic acid probes for harnessing nuclease activity as a biomarker of disease.

Tags

Nuclease ActivityNucleic Acid ProbesKinetic ScreeningBiomarkerNuclease DetectionOligonucleotide LibraryBacterial CultureNuclease AssayFluorometrySalmonellaE. Coli

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