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Immunofluorescence Labelling of Human and Murine Neutrophil Extracellular Traps in Paraffin-Embedded Tissue
JoVE Journal
Medicine
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JoVE Journal Medicine
Immunofluorescence Labelling of Human and Murine Neutrophil Extracellular Traps in Paraffin-Embedded Tissue

Immunofluorescence Labelling of Human and Murine Neutrophil Extracellular Traps in Paraffin-Embedded Tissue

DOI:
10.3791/60115-v

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07:17 min

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September 10, 2019

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1Microscopy Core Facility,Max Planck Institute for Infection Biology, 2Cellular Microbiology,Max Planck Institute for Infection Biology

Chapters

  • 00:04Title
  • 00:52Rehydration, Heat-induced Epitope Retrieval, Staining, Mounting, and Microscopic Analysis
  • 03:57Results: Immunofluorescence Labelling of Neutrophil Extracellular Traps in Paraffin-Embedded Tissue
  • 06:16Conclusion

Summary

Automatic Translation

Automatic Translation

Neutrophil extracellular traps (NETs) are three-dimensional structures generated by stimulated neutrophil granulocytes. It has become clear in recent years that NETs are involved in a wide variety of diseases. Detection of NETs in tissue may have diagnostic relevance, so standardized protocols for labelling NET components are required.

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