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Cochlear Surface Preparation in the Adult Mouse
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Neuroscience
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JoVE Journal Neuroscience
Cochlear Surface Preparation in the Adult Mouse

Cochlear Surface Preparation in the Adult Mouse

DOI:
10.3791/60299-v

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09:51 min

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November 06, 2019

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, , ,
1Department of Pathology and Laboratory Medicine,Medical University of South Carolina, 2MOE Key Laboratory of Developmental Genes and Human Disease, Institute of Life Sciences,Southeast University

Chapters

  • 00:05Title
  • 01:15Temporal Bone Extraction, Fixation, and Perfusion
  • 02:42Temporal Bone Decalcification
  • 03:31Cochlear Epithelium Microdissection
  • 05:56Cochlear Synapse Immunolabeling
  • 07:52Results: Representative Cochlear Preparation Imaging
  • 09:18Conclusion

Summary

Automatic Translation

Automatic Translation

This article presents a modified cochlear surface preparation method that requires decalcification and use of a cell and tissue adhesive to adhere the pieces of cochlear epithelia to 10 mm round cover slips for immunohistochemistry in adult mouse cochleae.

Tags

Keywords: Cochlear Surface PreparationImmunohistochemistryConfocal ImagingCochlear MicrodissectionCochlear EpitheliumHair Cell RegenerationTemporal Bone ExtractionCochlear FixationEDTA Decalcification

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