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Isolation and Differentiation of Primary Myoblasts from Mouse Skeletal Muscle Explants
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Chapters
- 00:05Title
- 00:46Collection and Processing of Muscle Tissue Explants
- 02:11Harvesting Outgrowing Myoblasts
- 04:16Expansion and Enrichment of Proliferating Myoblasts
- 05:54Results: Myoblasts and Myotubes
- 06:30Conclusion
Myoblasts are proliferating precursor cells that differentiate to form polynucleated myotubes and eventually skeletal muscle myofibers. Here, we present a protocol for efficient isolation and culture of primary myoblasts from young adult mouse skeletal muscles. The method enables molecular, genetic, and metabolic studies of muscle cells in culture.
