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Immunology and Infection

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JoVE Journal Immunology and Infection
Culture of Small Colony Variant of Pseudomonas aeruginosa and Quantitation of its Alginate
 

Culture of Small Colony Variant of Pseudomonas aeruginosa and Quantitation of its Alginate

Article DOI: 10.3791/60466-v • 09:06 min • February 22nd, 2020
February 22nd, 2020 •
*1, *2, 1,2,3
1Department of Biomedical Sciences, Joan C. Edwards School of Medicine, Marshall University, 2Progenesis Technologies LLC, Robert C. Byrd Biotechnology Science Center, 3Department of Pediatrics, Joan C. Edwards School of Medicine, Marshall University
* These authors contributed equally

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Summary
Automatic Translation

Here, we describe a growth condition to culture the small colony variant of Pseudomonas aeruginosa. We also describe two separate methods for the detection and quantitation of the exopolysaccharide alginate produced by P. aeruginosa using a traditional uronic acid carbazole assay and an alginate-specific monoclonal antibody (mAb) based ELISA.

Tags

Keywords: Pseudomonas Aeruginosa Small Colony Variant SCV Alginate ELISA Uronic Acid Carbazole Physiological Activation Salvage Pathway OD600
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