Journal
/
Bioengineering
/
Process Optimization using High Throughput Automated Micro-Bioreactors in Chinese Hamster Ovary Cell Cultivation
JoVE Journal
Bioengineering
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Bioengineering
Process Optimization using High Throughput Automated Micro-Bioreactors in Chinese Hamster Ovary Cell Cultivation

Process Optimization using High Throughput Automated Micro-Bioreactors in Chinese Hamster Ovary Cell Cultivation

DOI:
10.3791/60577-v

•

09:28 min

•

May 18, 2020

•

, , ,
1Institute for Technical Chemistry,Gottfried-Wilhelm-Leibniz Universität Hannover

Chapters

  • 00:04Introduction
  • 00:43Writing the Recipe in the Automated Micro-Bioreactor Software
  • 04:25Design of Experiment in the Automated Micro Bioreactor
  • 06:22Execution of the Experiment in the Work Packets and Analysis of Experimental Results
  • 07:25Results: DOE Experiment and Response Contour Plots
  • 08:44Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a detailed procedure to run a Design of Experiment in an automated micro-bioreactor followed by cell harvest and protein quantification using a Protein A column.

Tags

Process OptimizationHigh ThroughputAutomated Micro-bioreactorsChinese Hamster Ovary (CHO) Cell CultivationDesign Of Experiments (DOE)Data AnalysisPre-culture ProcedureCultivation ConditionsMedia AdditionInoculum AdditionFeed AdditionGlucose AdditionAnti-foam AdditionSoftware InterfaceAutomated Liquid Handling

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Developing Custom Chinese Hamster Ovary-host Cell Protein Assays using Acoustic Membrane Microparticle Technology

High Throughput Single-cell and Multiple-cell Micro-encapsulation

Using Tomoauto: A Protocol for High-throughput Automated Cryo-electron Tomography

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology

Automated Slide Scanning and Segmentation in Fluorescently-labeled Tissues Using a Widefield High-content Analysis System

Comprehensive Evaluation of the Effectiveness and Safety of Placenta-Targeted Drug Delivery Using Three Complementary Methods

Live Cell Analysis of Shear Stress on Pseudomonas aeruginosa Using an Automated Higher-Throughput Microfluidic System

Purification and Analytics of a Monoclonal Antibody from Chinese Hamster Ovary Cells Using an Automated Microbioreactor System

Generation of Dynamical Environmental Conditions using a High-Throughput Microfluidic Device

Automated Microbial Cultivation and Adaptive Evolution using Microbial Microdroplet Culture System (MMC)

Read Article