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Dissection and Lipid Droplet Staining of Oenocytes in Drosophila Larvae
JoVE Journal
Biology
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JoVE Journal Biology
Dissection and Lipid Droplet Staining of Oenocytes in Drosophila Larvae
DOI:

05:37 min

December 28, 2019

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Chapters

  • 00:04Title
  • 00:32Egg Laying
  • 01:13Starvation Treatment
  • 02:10Oenocyte Dissection
  • 03:10Lipid Droplet Staining
  • 04:02Mounting and Imaging
  • 04:41Results: Representative Lipid Droplet Staining
  • 05:06Conclusion

Summary

Automatic Translation

Presented here are detailed methods for the dissection and lipid droplet staining of oenocytes in Drosophila larvae using BODIPY 493/503, a lipid droplet-specific fluorescent dye.

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