Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Bioengineering / Isolation of Adult Human Dermal Fibroblasts from…
JoVE Journal
Bioengineering

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Bioengineering
Isolation of Adult Human Dermal Fibroblasts from Abdominal Skin and Generation of Induced Pluripotent Stem Cells Using a Non-Integrating Method
 

Isolation of Adult Human Dermal Fibroblasts from Abdominal Skin and Generation of Induced Pluripotent Stem Cells Using a Non-Integrating Method

Article DOI: 10.3791/60629-v • 10:52 min • January 19th, 2020
January 19th, 2020 •
*1, *1, 1, 1, 1, 1, 1, 1
1Department of Public Health, University of Naples Federico II
* These authors contributed equally

Chapters

Summary
Automatic Translation

Cell reprogramming requires the introduction of key genes, which regulate and maintain the pluripotent cell state. The protocol described enables the formation of induced pluripotent stem cells (iPSCs) colonies from human dermal fibroblasts without viral/integrating methods but using non-modified RNAs (NM-RNAs) combined with immune evasion factors reducing cellular defense mechanisms.

Tags

Keywords: Dermal Fibroblasts Induced Pluripotent Stem Cells Non-integrating Method Skin Punch Biopsy Regenerative Medicine Genetic Disorders Cancer Cell Isolation Cell Reprogramming Xeno-free Autologous IPS Cells Trypsin-EDTA TSS
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter