Journal
/
Medicine
/
Preparation of Peripheral Blood Mononuclear Cell Pellets and Plasma from a Single Blood Draw at Clinical Trial Sites for Biomarker Analysis
JoVE Journal
Medicine
This content is Free Access.
JoVE Journal Medicine
Preparation of Peripheral Blood Mononuclear Cell Pellets and Plasma from a Single Blood Draw at Clinical Trial Sites for Biomarker Analysis

Preparation of Peripheral Blood Mononuclear Cell Pellets and Plasma from a Single Blood Draw at Clinical Trial Sites for Biomarker Analysis

DOI:
10.3791/60776-v

•

07:40 min

•

March 20, 2021

•

, , , , , , ,
1Translational Medicine, R&D Oncology,AstraZeneca, 2Acerta Pharma, AstraZeneca Group, 3Translational Medicine, R&D Oncology,AstraZeneca

Chapters

  • 00:05Introduction
  • 01:08Whole Blood Irradiation for DNA Damage Response (DDR) Biomarker Expression Analysis
  • 03:35PBMC and Plasma Freezing
  • 04:45Western Blot Analysis
  • 05:43Results: Representative DNA Damage Assessment
  • 07:10Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol details clinically implementable preparation of high quality PBMC and plasma biosamples at the clinical trial site that can be used for translational biomarker analysis.

Tags

PBMCPlasmaBlood DrawBiomarker AnalysisClinical TrialPre-analytical VariabilityPharmacokineticsPharmacodynamicsDNA Damage ResponseRadiationCentrifugationCell SeparationBuffy Coat

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/60776?article_type=v

Rapid Fractionation and Isolation of Whole Blood Components in Samples Obtained from a Community-based Setting
check_url/60776?article_type=v

Orthotopic Implantation and Peripheral Immune Cell Monitoring in the II-45 Syngeneic Rat Mesothelioma Model
check_url/60776?article_type=v

In Vitro Assays to Assess Blood-brain Barrier Mesh-like Vessel Formation and Disruption
check_url/60776?article_type=v

Repeated Blood Collection from Tail Vein of Non-Anesthetized Rats with a Vacuum Blood Collection System
check_url/60776?article_type=v

Routine Screening Method for Microparticles in Platelet Transfusions
check_url/60776?article_type=v

Quantitative Analysis of Cellular Composition in Advanced Atherosclerotic Lesions of Smooth Muscle Cell Lineage-Tracing Mice
check_url/60776?article_type=v

Simultaneous Flow Cytometric Characterization of Multiple Cell Types Retrieved from Mouse Brain/Spinal Cord Through Different Homogenization Methods
check_url/60776?article_type=v

Monitoring PD-1-Blocking Antibodies Bound to T Cells Derived from a Drop of Peripheral Blood
check_url/60776?article_type=v

Evaluation of a Point-of-Care Testing Analyzer for Measuring Peripheral Blood Leukocytes
check_url/60776?article_type=v

Repetitive Blood Sampling from the Subclavian Vein of Conscious Rat

Read Article