Journal
/
Environment
/
Investigation of Xenobiotics Metabolism In Salix alba Leaves via Mass Spectrometry Imaging
JoVE Journal
Environment
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Environment
Investigation of Xenobiotics Metabolism In Salix alba Leaves via Mass Spectrometry Imaging

Investigation of Xenobiotics Metabolism In Salix alba Leaves via Mass Spectrometry Imaging

DOI:
10.3791/61011-v

•

09:21 min

•

June 15, 2020

•

, ,
1Plant Imaging and Mass Spectrometry (PIMS), Institut de biologie moléculaire des plantes, CNRS,Université de Strasbourg, 2Département mécanique,ICube Laboratoire des sciences de l’ingénieur, de l’informatique et de l’imagerie, UNISTRA/CNRS/ENGEES/INSA

Chapters

  • 00:04Introduction
  • 00:35Sample Preparation
  • 02:11Matrix Deposition
  • 02:59Data Acquisition
  • 05:22Data Processing
  • 07:46Results: In-situ Metabolic Processes in Response to Xenobiotics Exposure
  • 08:46Conclusion

Summary

Automatic Translation

Automatic Translation

This method uses mass spectrometry imaging (MSI) to understand metabolic processes in S. alba leaves when exposed to xenobiotics. The method allows the spatial localization of compounds of interest and their predicted metabolites within specific, intact tissues.

Tags

Xenobiotics MetabolismSalix AlbaMass Spectrometry ImagingMetabolite IdentificationCryo-microtomeSample PreparationMALDI-MSMatrix Deposition

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/61011?article_type=v

Mass Production of Genetically Modified Aedes aegypti for Field Releases in Brazil
check_url/61011?article_type=v

Optimize Flue Gas Settings to Promote Microalgae Growth in Photobioreactors via Computer Simulations
check_url/61011?article_type=v

An Aquatic Microbial Metaproteomics Workflow: From Cells to Tryptic Peptides Suitable for Tandem Mass Spectrometry-based Analysis
check_url/61011?article_type=v

Measuring Rates of Herbicide Metabolism in Dicot Weeds with an Excised Leaf Assay
check_url/61011?article_type=v

LC-MS Analysis of Human Platelets as a Platform for Studying Mitochondrial Metabolism
check_url/61011?article_type=v

Determination of Inorganic Arsenic in a Wide Range of Food Matrices using Hydride Generation – Atomic Absorption Spectrometry.
check_url/61011?article_type=v

Assessing the Particulate Matter Removal Abilities of Tree Leaves
check_url/61011?article_type=v

Detection of 3-Nitrotyrosine in Atmospheric Environments via a High-performance Liquid Chromatography-electrochemical Detector System
check_url/61011?article_type=v

Identifying Per- and Polyfluorinated Chemical Species with a Combined Targeted and Non-Targeted-Screening High-Resolution Mass Spectrometry Workflow
check_url/61011?article_type=v

Investigation of Plant Interactions Across Common Mycorrhizal Networks Using Rotated Cores

Read Article