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Assessment of Cellular Oxidation using a Subcellular Compartment-Specific Redox-Sensitive Green Fluorescent Protein
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Biochemistry
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JoVE Journal Biochemistry
Assessment of Cellular Oxidation using a Subcellular Compartment-Specific Redox-Sensitive Green Fluorescent Protein

Assessment of Cellular Oxidation using a Subcellular Compartment-Specific Redox-Sensitive Green Fluorescent Protein

DOI:
10.3791/61229-v

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06:10 min

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June 18, 2020

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, , ,
1Division of Radiation Health, Pharmaceutical Sciences,University of Arkansas for Medical Sciences, 2Department of Pharmaceutical Toxicology, Faculty of Pharmacy,Ege University

Chapters

  • 00:04Introduction
  • 00:46Cell Preparation
  • 01:33Adenoviral Redox Sensitive Green Fluorescent Protein (roGFP) Transduction
  • 02:46Cysteine/Cystine (CyS/CySS) Balance Acquisition
  • 04:01Results: Representative Cellular Oxidation Evaluation
  • 05:29Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes the assessment of subcellular compartment-specific redox status within the cell. A redox-sensitive fluorescent probe allows convenient ratiometric analysis in intact cells.

Tags

Subcellular CompartmentRedox-sensitive GFPOxidative StressRedox StateRatiometricNon-disruptiveTransductionFlow CytometryHydrogen Peroxide

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