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Pathological Analysis of Lung Metastasis Following Lateral Tail-Vein Injection of Tumor Cells
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JoVE Journal Cancer Research
Pathological Analysis of Lung Metastasis Following Lateral Tail-Vein Injection of Tumor Cells

Pathological Analysis of Lung Metastasis Following Lateral Tail-Vein Injection of Tumor Cells

DOI:
10.3791/61270-v

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08:54 min

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May 20, 2020

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, , ,
1Department of Radiation Oncology, Arthur G. James Comprehensive Cancer Center and Richard L. Solove Research Institute,The Ohio State University Medical Center, 2Department of Veterinary Biosciences, Comparative Pathology and Digital Imaging Shared Resource,The Ohio State University

Chapters

  • 00:04Introduction
  • 00:39Breast Cancer Cell Tail Vein Injection
  • 01:28Lateral Tail Vein Injection
  • 03:19Lung Tissue Fixation
  • 04:51Metastatic Lung Tumor Burden Analysis
  • 06:33Results: Representative Metastatic Lung Tumor Burden Analysis
  • 08:15Conclusion

Summary

Automatic Translation

Automatic Translation

Intravenous injection of cancer cells is often used in metastasis research, but the metastatic tumor burden can be difficult to analyze. Herein, we demonstrate a tail-vein injection model of metastasis and include a novel approach to analyze the resulting metastatic lung tumor burden.

Tags

Lung MetastasisTail-vein InjectionTumor CellsDigital Image AnalysisBreast Cancer CellsCell CultureMouse InjectionIn Vivo ImagingHistopathological Analysis

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