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An Automated Differential Nuclear Staining Assay for Accurate Determination of Mitocan Cytotoxicity
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Cancer Research
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JoVE Journal Cancer Research
An Automated Differential Nuclear Staining Assay for Accurate Determination of Mitocan Cytotoxicity

An Automated Differential Nuclear Staining Assay for Accurate Determination of Mitocan Cytotoxicity

DOI:
10.3791/61295-v

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07:58 min

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May 12, 2020

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, ,
1Department of BioSciences,Rice University

Chapters

  • 00:05Introduction
  • 00:54Cytotoxicity Assay Setup and Staining
  • 03:36Cytotoxicity Assay Data Acquisition
  • 05:43Results: Validation of Hoechst/PI Cytotoxicity Assay in Leukemia Cells
  • 07:08Conclusion

Summary

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Automatic Translation

The protocol describes a rapid, high-throughput, reliable, inexpensive, and unbiased assay for efficiently determining cellular viability. This assay is particularly useful when cells' mitochondria have been damaged, which interferes with other assays. The assay uses automated counting of cells stained with two nuclear dyes – Hoechst 33342 and propidium iodide.

Tags

Automated Differential Nuclear StainingMitocan CytotoxicityCell ViabilityHigh Throughput Drug ScreeningCytotoxicity DeterminationMitochondrial FunctionCell DensityTrypan Blue StainingCell SuspensionMulti-channel Pipette96-well PlateDMSO Concentration

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