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Fabrication of a Biomimetic Nano-Matrix with Janus Base Nanotubes and Fibronectin for Stem Cell Adhesion
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Bioengineering
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JoVE Journal Bioengineering
Fabrication of a Biomimetic Nano-Matrix with Janus Base Nanotubes and Fibronectin for Stem Cell Adhesion

Fabrication of a Biomimetic Nano-Matrix with Janus Base Nanotubes and Fibronectin for Stem Cell Adhesion

DOI:
10.3791/61317-v

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07:14 min

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May 10, 2020

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, , ,
1Department of Biomedical Engineering,University of Connecticut

Chapters

  • 00:00Introduction
  • 01:01Observation of Lyophilized Specimens
  • 02:29Absorption Spectra Measurements
  • 03:26In Vitro Biological Function Assay
  • 04:49Results: Self-assembled JBNT/FN Nanomatrices
  • 06:43Conclusion

Summary

Automatic Translation

Automatic Translation

The goal of this protocol is to show the assembly of a biomimetic nanomatrix (NM) with Janus base nanotubes (JBNTs) and fibronectin (FN). When co-cultured with human mesenchymal stem cells (hMSCs), the NMs exhibit excellent bioactivity in encouraging hMSCs adhesion.

Tags

Biomimetic Nano-matrixJanus Base NanotubesFibronectinStem Cell AdhesionExtracellular MatrixLyophilizedSpectrophotometer

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