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A Cell Culture Model for Studying the Role of Neuron-Glia Interactions in Ischemia
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Neuroscience
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JoVE Journal Neuroscience
A Cell Culture Model for Studying the Role of Neuron-Glia Interactions in Ischemia

A Cell Culture Model for Studying the Role of Neuron-Glia Interactions in Ischemia

DOI:
10.3791/61388-v

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11:36 min

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November 14, 2020

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, , , , , ,
1Centro de Investigação em Ciências da Saúde (CICS-UBI),Universidade da Beira Interior, 2Faculdade de Ciências da Saúde,Universidade da Beira Interior

Chapters

  • 00:00Introduction
  • 02:03Rat Embryo Cortex
  • 05:23Co-Culture System
  • 06:31Oxygen Glucose Deprivation
  • 07:57Culture Characterization
  • 09:19OGD Protocol
  • 10:47Conclusion

Summary

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Here, we present a simple approach using specific culture media that allows the establishment of neuron- and astrocyte-enriched cultures, or neuron-glia cultures from the embryonic cortex, with high yield and reproducibility.

Tags

Cell CultureNeuron-glia InteractionsIschemiaAstrocytesNeuronsOxygen And Glucose DeprivationRat Embryonic Cortex

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