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A Murine Model of Fetal Exposure to Maternal Inflammation to Study the Effects of Acute Chorioamnionitis on Newborn Intestinal Development
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A Murine Model of Fetal Exposure to Maternal Inflammation to Study the Effects of Acute Chorioamnionitis on Newborn Intestinal Development

A Murine Model of Fetal Exposure to Maternal Inflammation to Study the Effects of Acute Chorioamnionitis on Newborn Intestinal Development

DOI:
10.3791/61464-v

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08:50 min

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June 24, 2020

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, , , , ,
1Division of Neonatology, Stead Family Department of Pediatrics,University of Iowa, 2Division of Maternal Fetal Medicine, Department of Obstetrics & Gynecology,University of Iowa, 3Division of Neonatology, Department of Pediatrics,Vanderbilt University, 4Department of Microbiology & Immunology,University of Iowa

Chapters

  • 00:04Introduction
  • 00:52Establishment of FEMI in Pregnant Mice
  • 02:06Delivery and Care of Offspring and Intestinal Harvesting
  • 03:03Intestinal Injury Scoring
  • 04:11Quantification of Paneth and Goblet Cells
  • 06:35Results: Intestinal Injury of Newborn Mice Induced by FEMI
  • 08:22Conclusion

Summary

Automatic Translation

Automatic Translation

We developed a model of chorioamnionitis to simulate fetal exposure to maternal inflammation (FEMI) without complications of live organisms to examine the effects of FEMI on development of the offspring’s intestinal tract. This allows for study of mechanistic causes for development of intestinal injury following chorioamnionitis.

Tags

Keywords: Fetal ExposureMaternal InflammationFEMINecrotizing EnterocolitisNECChorioamnionitisLPS InjectionPregnant MouseIntestinal DevelopmentRNA QuantificationHistology

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