Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / Analysis of SEC-SAXS data via EFA deconvolution…
JoVE Journal
Biochemistry

This content is Open Access.

JoVE Journal Biochemistry
Analysis of SEC-SAXS data via EFA deconvolution and Scatter
 

Analysis of SEC-SAXS data via EFA deconvolution and Scatter

Article DOI: 10.3791/61578-v • 10:59 min • January 28th, 2021
January 28th, 2021 •
*1, 2, 3, *4
1European Synchrotron Radiation Facility Structural Biology Group, Structural Biology Group, 2Institut de Biologie Structurale, University Grenoble Alpes, CEA, CNRS, 3Diamond Light Source, 4Laboratoire de Physiologie Cellulaire and Végétale, Université Grenoble Alpes/CNRS/CEA/INRA/BIG
* These authors contributed equally

Chapters

Summary
Automatic Translation

SEC-BioSAXS measurements of biological macromolecules are a standard approach for determining solution structure of macromolecules and their complexes. Here, we analyze SEC-BioSAXS data from two types of commonly encountered SEC traces—chromatograms with fully resolved and partially resolved peaks. We demonstrate the analysis and deconvolution using scatter and BioXTAS RAW.

Tags

Keywords: SEC-SAXS EFA Deconvolution Scatter Vaccinia Virus DNA Polymerase Background Subtraction Size Exclusion Chromatography Monodispersity Macromolecule Macromolecular Complex Deconvolution Data Analysis
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter