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Horizontal Hippocampal Slices of the Mouse Brain
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Neuroscience
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JoVE Journal Neuroscience
Horizontal Hippocampal Slices of the Mouse Brain

Horizontal Hippocampal Slices of the Mouse Brain

DOI:
10.3791/61753-v

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08:59 min

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September 22, 2020

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, , , ,
1Laboratory of Endometrium, Endometriosis and Reproductive Medicine, Department of Development and Regeneration,KU Leuven, 2Laboratory of Ion Channel Research, VIB-KU Leuven Center for Brain and Disease Research, Leuven, Belgium and Department of Molecular Medicine,KU Leuven

Chapters

  • 00:04Introduction
  • 00:47Artificial Cerebrospinal Fluid (ACSF) and High-Sucrose Slice Solution Preparation
  • 02:09Brain Dissection Preparation
  • 03:00Brain Positioning and Dissection
  • 05:02Horizontal Brain Slicing
  • 06:17Results: Representative Mouse Horizontal Hippocampal Brain Slice Fiber Pathway Analyses
  • 08:18Conclusion

Summary

Automatic Translation

Automatic Translation

This article aims to describe a systematic protocol to obtain horizontal hippocampal brain slices in mice. The objective of this methodology is to preserve the integrity of hippocampal fiber pathways, such as the perforant path and the mossy fiber tract to assess dentate gyrus related neurological processes.

Tags

Horizontal Hippocampal SlicesMouse BrainNeurological ChangesBrain PathologiesDentate GyrusACSFHigh-sucrose Slice SolutionVibratomeBrain DissectionMale Mouse

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