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Direct Bioprinting of 3D Multicellular Breast Spheroids onto Endothelial Networks
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Bioengineering
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JoVE Journal Bioengineering
Direct Bioprinting of 3D Multicellular Breast Spheroids onto Endothelial Networks

Direct Bioprinting of 3D Multicellular Breast Spheroids onto Endothelial Networks

DOI:
10.3791/61791-v

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06:07 min

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November 02, 2020

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,
1Department of Biology,Drexel University, 2Fischell Department of Bioengineering,University of Maryland

Chapters

  • 00:05Introduction
  • 00:50Breast Epithelial Spheroid Formation
  • 02:06Human Umbilical Vein Endothelial Cell (HUVEC) Network Formation
  • 03:12Breast Epithelial Spheroid Bioprinting onto Pre-Formed HUVEC Networks
  • 04:24Results: Representative Breast Epithelial Spheroid and HUVEC Network Imaging
  • 05:19Conclusion

Summary

Automatic Translation

Automatic Translation

The goal of this protocol is to directly bioprint breast epithelial cells as multicellular spheroids onto pre-formed endothelial networks to rapidly create 3D breast-endothelial co-culture models which can be used for drug screening studies.

Tags

Keywords: 3D BioprintingBreast SpheroidsEndothelial NetworksMulticellular ModelsPrecision Medicine3D Cell CultureMCF 10A CellsHUVECCell TrackingMatrix CoatingCell ResuspensionCell Culture Protocols

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