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Chapters
- 00:04Introduction
- 00:42Femoral Cannulation
- 01:51Thoracotomy
- 02:58Pressure Volume Loop (PVL) Measurement
- 04:15PVL Parallel-Conductance Calibration
- 04:54PVL Conductance-to-Volume Calibration
- 05:32Results: Representative PVL Analysis
- 07:11Conclusion
Here we describe a cardiac pressure-volume loop analysis under increasing doses of intravenously infused isoproterenol to determine the intrinsic cardiac function and the β-adrenergic reserve in mice. We use a modified open-chest approach for the pressure-volume loop measurements, in which we include ventilation with positive end-expiratory pressure.
