Journal
/
Biology
/
Single Myofiber Culture Assay for the Assessment of Adult Muscle Stem Cell Functionality Ex Vivo
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Single Myofiber Culture Assay for the Assessment of Adult Muscle Stem Cell Functionality Ex Vivo

Single Myofiber Culture Assay for the Assessment of Adult Muscle Stem Cell Functionality Ex Vivo

DOI:
10.3791/62257-v

•

09:19 min

•

February 15, 2021

•

, , , , ,
1Leibniz Institute on Aging, Fritz-Lipmann-Institute

Chapters

  • 00:04Introduction
  • 00:50Preparing Pipettes for Trituration of the Collagenase-Digested Muscles
  • 01:54EDL Muscle Isolation and Collagenase Digestion
  • 03:19Myofiber Dissociation and Culture
  • 04:20siRNA Transfection, Fixation, and IF Staining
  • 06:31Results: Culture and Transfection of Murine EDL Muscle-Derived Single Myofibres
  • 08:34Conclusion

Summary

Automatic Translation

Automatic Translation

In this protocol an in vitro culturing and functional analysis method for muscle stem cells is described, which preserves most of their interactions with their endogenous niche.

Tags

Muscle Stem CellsSingle Myofiber CultureEx VivoNicheSiRNA TransfectionPasteur PipettesHorse SerumEthanolTibialis AnteriorEDL MuscleDigestionIsolation MediumMyofibers

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/62257?article_type=v

Method for Culture of Early Chick Embryos ex vivo (New Culture)
check_url/62257?article_type=v

Adult and Embryonic Skeletal Muscle Microexplant Culture and Isolation of Skeletal Muscle Stem Cells
check_url/62257?article_type=v

Lentiviral-mediated Knockdown During Ex Vivo Erythropoiesis of Human Hematopoietic Stem Cells
check_url/62257?article_type=v

Ex Vivo Assessment of Contractility, Fatigability and Alternans in Isolated Skeletal Muscles
check_url/62257?article_type=v

Isolation and Culture of Individual Myofibers and their Satellite Cells from Adult Skeletal Muscle
check_url/62257?article_type=v

A Novel Ex vivo Culture Method for the Embryonic Mouse Heart
check_url/62257?article_type=v

Intravital Microscopy of the Microcirculation in the Mouse Cremaster Muscle for the Analysis of Peripheral Stem Cell Migration
check_url/62257?article_type=v

Isolation, Culture, and Transplantation of Muscle Satellite Cells
check_url/62257?article_type=v

Isolation of Blood-vessel-derived Multipotent Precursors from Human Skeletal Muscle
check_url/62257?article_type=v

An Ex vivo Culture System to Study Thyroid Development

Read Article