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Preparation of Sample Support Films in Transmission Electron Microscopy using a Support Floatation Block
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Preparation of Sample Support Films in Transmission Electron Microscopy using a Support Floatation Block

Preparation of Sample Support Films in Transmission Electron Microscopy using a Support Floatation Block

DOI:
10.3791/62321-v

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06:10 min

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April 08, 2021

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, ,
1Section of Structural & Synthetic Biology, Department of Infectious Diseases, Faculty of Medicine,Imperial College London, South Kensington Campus

Chapters

  • 00:04Introduction
  • 00:47Preparation of Negatively Stained Samples with Amorphous Carbon Support
  • 02:21Preparation of Graphene Oxide-coated TEM Grids
  • 03:27Preparation of Samples on Monolayer-graphene Films Using the Flotation Block
  • 04:44Results: Examples of Sample Support Films Prepared Using the Floatation Block
  • 05:34Conclusion

Summary

Automatic Translation

Automatic Translation

Sample preparation for cryo-electron microscopy (cryo-EM) is a significant bottleneck in the structure determination workflow of this method. Here, we provide detailed methods for using an easy-to-use, three-dimensionally printed block for the preparation of support films to stabilize samples for transmission EM studies.

Tags

Keywords: Transmission Electron MicroscopySample PreparationCryo-EMSupport FilmsFlotation BlockGraphene OxideNegative Staining

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