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Size Exclusion Chromatography to Analyze Bacterial Outer Membrane Vesicle Heterogeneity
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Chapters
- 00:04Introduction
- 00:53S-1000 Column Packing
- 02:22Sample Loading and Fraction Collection
- 03:52Sample Analysis
- 05:51Results: Analysis of Lipid and Toxin Contents of the Collected Fractions
- 06:51Conclusion
Bacterial vesicles play important roles in pathogenesis and have promising biotechnological applications. The heterogeneity of vesicles complicates analysis and use; therefore, a simple, reproducible method to separate varying sizes of vesicles is necessary. Here, we demonstrate the use of size exclusion chromatography to separate heterogeneous vesicles produced by Aggregatibacter actinomycetemcomitans.
