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Microtubule Plus-End Dynamics Visualization in Huntington’s Disease Model based on Human Primary Skin Fibroblasts
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JoVE Journal Bioengineering
Microtubule Plus-End Dynamics Visualization in Huntington’s Disease Model based on Human Primary Skin Fibroblasts

Microtubule Plus-End Dynamics Visualization in Huntington’s Disease Model based on Human Primary Skin Fibroblasts

DOI:
10.3791/62963-v

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10:38 min

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January 08, 2022

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, , , , ,
1A.N. Belozersky Institute of Physico-Chemical Biology,Lomonosov Moscow State University, 2Faculty of Bioengineering and Bioinformatics,Lomonosov Moscow State University, 3Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 4Center for Precision Genome Editing and Genetic Technologies for Biomedicine,Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency

Chapters

  • 00:05Introduction
  • 00:44Protocol
  • 01:02Obtaining a Primary Culture of Human Skin Fibroblasts
  • 02:14Cell Cultivation
  • 04:45Transfection
  • 06:46Preparing for Imaging
  • 08:08Setting the Imaging Parameters
  • 09:45Results
  • 10:13Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol is dedicated to the microtubule plus-end visualization by EB3 protein transfection to study their dynamic properties in primary cell culture. The protocol was implemented on human primary skin fibroblasts obtained from Huntington's disease patients.

Tags

Microtubule DynamicsEB3 ProteinHuntington’s DiseaseHuman Primary Skin FibroblastsCell CultureTransfectionFluorescent LabelingMicroscopy

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