Journal
/
Biochemistry
/
Sample Preparation using a Lipid Monolayer Method for Electron Crystallographic Studies
JoVE Journal
Biochemistry
Author Produced
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
Sample Preparation using a Lipid Monolayer Method for Electron Crystallographic Studies

Sample Preparation using a Lipid Monolayer Method for Electron Crystallographic Studies

DOI:
10.3791/63015-v

•

04:22 min

•

November 20, 2021

•

, , , ,
1School of Molecular Sciences,Arizona State University, 2Center for Applied Structural Discovery, Biodesign Institute,Arizona State University, 3Eyring Materials Center,Arizona State University, 4Department of Microbiology and Immunology,The Pennsylvania State University

Summary

Automatic Translation

Automatic Translation

Lipid monolayers have been used as a foundation for forming two-dimensional (2D) protein crystals for structural studies for decades. They are stable at the air-water interface and can serve as a thin supporting material for electron imaging. Here we present the proven steps on preparing lipid monolayers for biological studies.

Tags

Keywords: Lipid MonolayerElectron Crystallography2D CrystallizationMembrane ProteinsProtein BindingSample PreparationTeflon PlateBuffer ReservoirHumidity ControlCarbon-coated EM Grid

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/63015?article_type=v

Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro
check_url/63015?article_type=v

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions
check_url/63015?article_type=v

A Plasma Sample Preparation for Mass Spectrometry using an Automated Workstation
check_url/63015?article_type=v

Sample Preparation for Probe Electrospray Ionization Mass Spectrometry
check_url/63015?article_type=v

TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis
check_url/63015?article_type=v

Workflow and Tools for Crystallographic Fragment Screening at the Helmholtz-Zentrum Berlin
check_url/63015?article_type=v

A Sample Preparation Pipeline for Microcrystals at the VMXm Beamline
check_url/63015?article_type=v

Preparation of Sample Support Films in Transmission Electron Microscopy using a Support Floatation Block
check_url/63015?article_type=v

Large-Scale Multi-Omics Genome-Wide Association Studies (Mo-GWAS): Guidelines for Sample Preparation and Normalization
check_url/63015?article_type=v

Preparation of High-Temperature Sample Grids for Cryo-EM

Read Article