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A Porcine Heterotopic Heart Transplantation Protocol for Delivery of Therapeutics to a Cardiac Allograft
JoVE Journal
Medicine
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JoVE Journal Medicine
A Porcine Heterotopic Heart Transplantation Protocol for Delivery of Therapeutics to a Cardiac Allograft
DOI:

08:30 min

February 14, 2022

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Chapters

  • 00:04Introduction
  • 01:00Median Sternotomy of the Donor Pig
  • 01:56Cardiac Arrest and Cardiectomy of the Donor Pig
  • 03:39Back-Table Preparation of the Donor Heart and Reanimating the Heart
  • 04:47Preparation of the Recipient and Laparotomy with Vascular Exposure
  • 05:45Heterotopic Implantation of the Cardiac Graft and Closure of the Laparotomy
  • 06:49Results: Analysis of Perfusion Parameters and Transplanted Heart
  • 07:36Conclusion

Summary

Automatic Translation

We present a protocol for utilizing a normothermic ex vivo sanguinous perfusion system for the delivery of therapeutics to an entire cardiac allograft in a porcine heterotopic heart transplant model.

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