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Bioengineering

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JoVE Journal Bioengineering
Design to Implementation Study for Development and Patient Validation of Paper-Based Toehold Switch Diagnostics
 

Design to Implementation Study for Development and Patient Validation of Paper-Based Toehold Switch Diagnostics

Article DOI: 10.3791/63223-v • 10:42 min • June 17th, 2022
June 17th, 2022 •
*1, *1,2, *1, 3,4, 1, 1, 1, 1, 1, 3,4, 2, 1,5
1Leslie Dan Faculty of Pharmacy, University of Toronto, 2Laboratory of Virology and Experimental Therapy (LAVITE), Department of Virology, Aggeu Magalhães Institute (IAM), Oswaldo Cruz Foundation (Fiocruz), 3Department of Biomedical Engineering, Boston University, 4Molecular Biology, Cell Biology & Biochemistry Program, Graduate School of Arts and Sciences, Boston University, 5Department of Mechanical and Industrial Engineering, University of Toronto
* These authors contributed equally

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Summary
Automatic Translation

Access to decentralized, low-cost, and high-capacity diagnostics that can be deployed into the community for decentralized testing is critical for combating global health crises. This manuscript describes how to build paper-based diagnostics for viral RNA sequences that can be detected with a portable optical reader.

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Keywords: Paper-based Diagnostics Toehold Switch Gene Circuit Nucleic Acid Detection Cell-free Diagnostics Zika Virus MATLAB Design Software NCBI BLAST PCR Agarose Gel Electrophoresis
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