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Journal / Neuroscience / Demonstrating Hairy and Glabrous Skin Innervation …
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JoVE Journal Neuroscience
Demonstrating Hairy and Glabrous Skin Innervation in a 3D Pattern Using Multiple Fluorescent Staining and Tissue Clearing Approaches
 

Demonstrating Hairy and Glabrous Skin Innervation in a 3D Pattern Using Multiple Fluorescent Staining and Tissue Clearing Approaches

Article DOI: 10.3791/63807-v • 05:23 min • May 20th, 2022
May 20th, 2022 •
*1, *1, *1, 1, 1, 1, 1, 1, 1, 1, 1
1Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences
* These authors contributed equally

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Summary
Automatic Translation

The thickness of tissue sections limited the morphological study of the skin innervation. The present protocol describes a unique tissue clearing technique to visualize cutaneous nerve fibers in thick 300 µm tissue sections under confocal microscopy.

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Keywords: Tissue Clearing Fluorescent Staining Skin Innervation CGRP LYVE-1 3D Imaging Confocal Microscopy Tissue Sectioning Tissue Embedding Tissue Clearing Reagent
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