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A Novel High-Throughput Ex Vivo Ovine Skin Wound Model for Testing Emerging Antibiotics
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Immunology and Infection
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JoVE Journal Immunology and Infection
A Novel High-Throughput Ex Vivo Ovine Skin Wound Model for Testing Emerging Antibiotics

A Novel High-Throughput Ex Vivo Ovine Skin Wound Model for Testing Emerging Antibiotics

DOI:
10.3791/64041-v

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08:30 min

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September 16, 2022

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, ,
1Sheffield Collaboratorium for Antimicrobial Resistance and Biofilms (SCARAB),University of Sheffield, 2Department of Chemical and Biological Engineering,University of Sheffield

Chapters

  • 00:05Introduction
  • 00:53Sterilization and Preparation of the Head
  • 03:06Maintenance of Skin Samples
  • 04:18Infection of Skin Samples
  • 05:27Determination of the Bacterial Load
  • 06:32Results: An Ex Vivo Ovine Wounded Skin Model Infected with Staphylococcus aureus
  • 07:42Conclusion

Summary

Automatic Translation

Automatic Translation

The protocol describes a step-by-step method to set up an ex vivo ovine wounded skin model infected with Staphylococcus aureus. This high-throughput model better simulates infections in vivo compared with conventional microbiology techniques and presents researchers with a physiologically relevant platform to test the efficacy of emerging antimicrobials.

Tags

Ovine Skin Wound ModelHigh-throughput Ex VivoAntimicrobial EfficacyTopical AntimicrobialsWound InfectionsContaminationAseptic TechniqueSkin SampleChlorine Dioxide SolutionSterile PBSAir-liquid Interface

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