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Experimental Melanoma Immunotherapy Model Using Tumor Vaccination with a Hematopoietic Cytokine
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Chapters
- 00:04Introduction
- 00:56Preparation of Cultured Tumor Cells for Implantation
- 02:33Tumor Implantation
- 03:19Vaccine Preparation and Injection of Flt3L-Expressing B16 F10 (B16-Flt3) Cells
- 04:20Intratumoral Immune Cell Isolation
- 06:59Results: Using a Cell-Based Tumor Vaccine Expressing Flt3 Model to Study Intratumoral Immune Cell Infiltrates
- 08:45Conclusion
The protocol presents a cancer immunotherapy model using cell-based tumor vaccination with Flt3L-expressing B16-F10 melanoma. This protocol demonstrates the procedures, including preparation of cultured tumor cells, tumor implantation, cell irradiation, measurement of tumor growth, isolation of intratumoral immune cells, and flow cytometry analysis.
