Journal
/
/
Zygote Microinjection for Creating Gene Cassette Knock-in and Flox Alleles in Mice
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal Developmental Biology
Zygote Microinjection for Creating Gene Cassette Knock-in and Flox Alleles in Mice
DOI:

08:48 min

June 24, 2022

, , , , , , ,

Chapters

  • 00:04Introduction
  • 01:20Obtaining Mice Zygotes by Natural Mating
  • 02:29Preparation of the Microinjection Needle and Zygote Microinjection
  • 06:46Embryo Transfer into the Oviduct
  • 07:15Results: Analysis of Production Efficiency of Microinjection
  • 08:16Conclusion

Summary

Automatic Translation

The present protocol describes zygote microinjection of CRISPR-Cas9 and donor DNA to efficiently produce gene cassette knock-in and floxed mice.

Related Videos

Read Article