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Real-Time Quantitative Measurement of Tumor Cell Migration and Invasion Following Synthetic mRNA Transfection
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Cancer Research
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JoVE Journal Cancer Research
Real-Time Quantitative Measurement of Tumor Cell Migration and Invasion Following Synthetic mRNA Transfection

Real-Time Quantitative Measurement of Tumor Cell Migration and Invasion Following Synthetic mRNA Transfection

DOI:
10.3791/64274-v

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11:00 min

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June 23, 2023

•

,
1Children’s Mercy Research Institute,Children’s Mercy Kansas City, 2Department of Pediatrics,University of Missouri-Kansas City School of Medicine

Chapters

  • 00:04Introduction
  • 00:56Synthesis of mRNA
  • 03:57Extracellular Matrix (ECM) Gel Coating of the Cell Invasion and Migration (CIM) Plates
  • 04:38Electroporation of the Tumor Cells
  • 05:40Setting Up the Real‐Time Cell Analyzer, the Program, and the CIM Plates
  • 07:29Real‐Time Cell Analysis and Data Export
  • 08:23Results: Tumor Cell Migration and Invasion
  • 10:17Conclusion

Summary

Automatic Translation

Automatic Translation

Many upregulated genes stimulate tumor cell migration and invasion, leading to poor prognosis. Determining which genes regulate tumor cell migration and invasion is critical. This protocol presents a method for investigating the effects of the increased expression of a gene on the migration and invasion of tumor cells in real time.

Tags

Tumor Cell MigrationTumor Cell InvasionReal-time Quantitative MeasurementSynthetic MRNA TransfectionImpedance-based Real-time Cell AnalysisGene ExpressionMetastasisCancer TherapyRNA SynthesisLithium Chloride PrecipitationCappingPoly-A Tailing

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