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Visualizing Early Infection Sites of Rice Blast Disease (Magnaporthe oryzae) on Barley (Hordeum vulgare) Using a Basic Microscope and a Smartphone
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Chapters
- 00:04Introduction
- 00:40Infecting Barley (Hordeum vulgare) with Rice Blast Fungus (Magnaporthe oryzae)
- 03:11Staining the Infected Leaf Sheaths
- 04:12Mounting and Imaging of the Stained Leaf Sheaths
- 05:51Results: Infected and Stained Sheaths Imaging
- 06:57Conclusion
This is a straightforward protocol of a barley leaf sheath assay using minimal reagents and common laboratory equipment (including a basic smartphone). The purpose is to visualize the early infection process of blast disease in labs without access to advanced microscopy equipment.
