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A Behavioral Assay to Measure Responsiveness of Zebrafish to Changes in Light Intensities
JoVE Journal
Biology
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JoVE Journal Biology
A Behavioral Assay to Measure Responsiveness of Zebrafish to Changes in Light Intensities
DOI:

09:39 min

October 03, 2008

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Chapters

  • 00:00Introduction
  • 01:15Plating Individual Fish into a 96-well Plate
  • 02:41Survey of the Recording Apparatus
  • 04:19Alignment of the 96-well Plate with the Computer Grid of the Video-Tracking Software
  • 06:51Analyzing the Visual Motor Response Data
  • 09:05Conclusion

Summary

Automatic Translation

We developed the Visual-Motor Response to quantitate the motor output of larval zebrafish in response to light increments and decrements. We also examined zebrafish vision mutants, including the no optokinetic response (nrc) mutants, which were thought to be completely blind when tested by another vision assay, the optokinetic reflex.

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