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High-Throughput Cellular Profiling of Targeted Protein Degradation Compounds Using HiBiT CRISPR Cell Lines
This content is Free Access.
Chapters
- 00:04Introduction
- 00:50Endpoint Degradation Studies with HiBiT CRISPR Target Proteins in Lytic Format with Optional Cell Viability Fluorescence Analysis
- 03:07Results: Degradation Compound Analysis in a HiBiT CRISPR Cell Line
- 04:51Conclusion
This protocol describes the quantitative luminescent detection of protein degradation kinetics in living cells that have been engineered using CRISPR/Cas9 to express antibody free endogenous protein detection tag fused to a target protein. Detailed instructions for calculating and obtaining quantitative degradation parameters, rate, Dmax, DC50, and Dmax50 are included.
