High-Throughput Screening Analysis for 4B Cysteine Peptidase Autophagy Inhibitor

June 30, 2023

Author Spotlight: A Selective Luciferase-Based Assay for Monitoring ATG4B 27 Activity in Cells

Autophagy Protease Tag-Compound Addition and Cellular Supernatant Harvest for Drug Screening

Luciferase Assay for the Detection of Autophagy Protease ATG4B

Image Acquisition and Analysis for the Detection of Autophagy Protease ATG4B Using Luciferase Assay

Denise R. B. Pilger, Christin Luft, Robin Ketteler²

¹Laboratory for Molecular Cell Biology,University College London, ²Department of Human Medicine,Medical School Berlin

Summary

Here, we describe a detailed protocol for the use of a luciferase-based reporter assay in a semi-automated, high-throughput screening format.

Article