Quantitative Image Analysis to Measure the mtDNA and Gene Expression Levels in the Transfected HeLa Cells

Quantitative Image Analysis to Measure the mtDNA and Gene Expression Levels in the Transfected HeLa Cells (Video) | JoVE

Published: May 05, 2023

doi:

10.3791/200044

Lukasz S. Borowski², Karolina Kasztelan³, Jolanta Czerwinska-Kostrzewska², Roman J. Szczesny

¹Faculty of Biology, Institute of Genetics and Biotechnology,University of Warsaw, ²Institute of Biochemistry and Biophysics,Polish Academy of Sciences, ³International Institute of Molecular and Cell Biology in Warsaw

Tags

Quantitative Image Analysis MtDNA Levels Gene Expression Transfected HeLa Cells Fluorescence Imaging Background Correction Image Segmentation BrdU Mitochondrial DNA Quantitative Real-time PCR Dideoxycytidine DDC TFAM TWINKLE Helicase POLG Mitochondrial DNA Polymerase Gamma

Play Video

PDF

DOI

Cite This Article

Quantitative Image Analysis to Measure the mtDNA and Gene Expression Levels in the Transfected HeLa Cells. J. Vis. Exp. (Pending Publication), e200044, doi:10.3791/200044 (2023).

Quantitative Image Analysis to Measure the mtDNA and Gene Expression Levels in the Transfected HeLa Cells (Video) | JoVE

Tags

Play Video

Cite This Article

View Video

✖

To prove you're not a robot, please enter the text in the image below