Journal
/
Biochemistry
/
High-Throughput Quantification of the Synthesis and Distribution of mtDNA
/
Quantitative Image Analysis to Measure the mtDNA and Gene Expression Levels in the Transfected HeLa Cells (Video) | JoVE
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
Quantitative Image Analysis to Measure the mtDNA and Gene Expression Levels in the Transfected HeLa Cells

Quantitative Image Analysis to Measure the mtDNA and Gene Expression Levels in the Transfected HeLa Cells (Video) | JoVE

High-Throughput Quantification of the Synthesis and Distribution of mtDNA

DOI:
10.3791/200044-v

•

03:02 min

•

May 05, 2023

•

, , ,
1Faculty of Biology, Institute of Genetics and Biotechnology,University of Warsaw, 2Institute of Biochemistry and Biophysics,Polish Academy of Sciences, 3International Institute of Molecular and Cell Biology in Warsaw

Tags

Quantitative Image AnalysisMtDNA LevelsGene ExpressionTransfected HeLa CellsFluorescence ImagingBackground CorrectionImage SegmentationBrdUMitochondrial DNAQuantitative Real-time PCRDideoxycytidineDDCTFAMTWINKLE HelicasePOLGMitochondrial DNA Polymerase Gamma

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/200044?article_type=v

High-Throughput Image-Based Quantification of Mitochondrial DNA Synthesis and Distribution (Video) | JoVE

check_url/200044?article_type=v

Preparation of siRNA Mixture for Mitochondrial DNA Synthesis (Video) | JoVE

check_url/200044?article_type=v

Cell Transfection and 5-Bromo-2'-Deoxyuridine (BrdU) Incorporation in HeLa Cells (Video) | JoVE

check_url/200044?article_type=v

5-Bromo-2'-Deoxyuridine (BrdU) Labeling of Mitochondrial DNA in HeLa Cells (Video) | JoVE

check_url/200044?article_type=v

HeLa Cell Fixation with Hoechst 33342 and Blocking (Video) | JoVE

check_url/200044?article_type=v

Addition of Primary and Secondary Antibodies to the Transfected HeLa Cells to Monitor the mtDNA Distribution (Video) | JoVE

check_url/200044?article_type=v

Quantitative Image Analysis to Measure the mtDNA and Gene Expression Levels in the Transfected HeLa Cells (Video) | JoVE

Read Article